The USPTO published application US20260092264A1, disclosing a method for purifying heparan-N-sulfatase from solutions containing impurities. The method combines multi-mode chromatography to obtain an eluate, followed by caprylate precipitation to produce a purified supernatant. The claimed invention is classified under CPC codes C12N 9/14, C07K 1/165, C07K 1/30, C07K 1/34, and C12Y 310/01001, indicating applications in enzyme chemistry and protein purification.

Patent applications do not impose compliance obligations on industry. For biopharmaceutical manufacturers developing heparan-N-sulfatase-based therapeutics, this publication represents potential prior art that could affect freedom-to-operate assessments. Research and development teams working on enzyme purification processes should review the disclosed methods for potential licensing opportunities or to assess patent landscape implications.