NOVEL PROCESS FOR PURIFYING HEPARAN-N-SULFATASE
Application
US20260092264A1
Kind: A1
Apr 02, 2026
Inventors
Kwanyoung KO, Dasom KIM, Sulgi YOO, Geunhye YEO, Minjung KIM, Dong Kyu JIN
Abstract
The present invention is related to a method for purifying heparan-N-sulfatase from a heparan-N-sulfatase-containing solution including at least one impurity, the method comprising performing multi-mode chromatography (MMC) to obtain an eluate; and performing caprylate precipitation to obtain a supernatant. The method according to the present invention is capable of very efficiently removing HCP (host cell proteins) and of greatly improving the purity and stability of purified heparan-N-sulfatase.
CPC Classifications
C12N 9/14
C07K 1/165
C07K 1/30
C07K 1/34
C12Y 310/01001
Filing Date
2023-09-18
Application No.
19110710