This document is a publication of a patent application (US20260085290A1) filed by DAI NIPPON PRINTING CO., LTD. The application describes a novel method for producing feeder cells and cell sheets derived from pluripotent stem cells. These feeder cells are designed to facilitate the maintenance and proliferation of cells that are typically challenging to culture using standard two-dimensional methods. The invention specifically involves deriving fibroblast-like cells from an endoderm- and mesoderm-derived cell population within the intestinal structure.

As this is a patent application, it does not impose direct regulatory obligations or compliance deadlines on entities. However, it represents a new development in biotechnology that may impact intellectual property strategies for companies in the pharmaceutical, biotechnology, and medical device sectors. Companies involved in stem cell research, cell culture, or regenerative medicine should be aware of this filing for potential licensing or competitive intelligence purposes. The application was filed on September 6, 2023.