Single Cell Library Construction with Tissue Location Data
Summary
Beijing SeekGene Biosciences Co., Ltd. filed US Patent Application US20260109969A1 for a method of constructing a single cell library containing tissue location information and an associated sequencing method, published April 23, 2026. The application names eight inventors and describes a five-step process enabling simultaneous analysis of spatial location and genetic information at the single cell level. Patent prosecution would determine whether claims covering the chip-based binding, tissue dissociation, and high-throughput library construction method are allowed.
“According to the method, spatial location information of a single cell can be tested while analyzing genetic information on a single cell level.”
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GovPing monitors USPTO Patent Applications - Biotech (C12N) for new healthcare & life sciences regulatory changes. Every update since tracking began is archived, classified, and available as free RSS or email alerts — 264 changes logged to date.
What changed
Beijing SeekGene Biosciences Co., Ltd. seeks patent protection for a method that constructs single cell libraries retaining tissue spatial location information, enabling simultaneous transcriptome and spatial genomic analysis. The five-step method involves providing a chip with location-encoded nucleic acid molecules, binding tissue slices to the chip, dissociating bound tissue into single cell suspension, and constructing both transcriptome and spatial barcoded libraries for sequencing.
Biotechnology and life sciences companies engaged in single cell genomics, spatial transcriptomics, or drug discovery research should monitor this application's prosecution to assess potential freedom-to-operate considerations. Competitors developing similar spatial profiling technologies may need to evaluate whether the disclosed methods and chip configurations could overlap with their own research programs or existing patent portfolios.
Archived snapshot
Apr 23, 2026GovPing captured this document from the original source. If the source has since changed or been removed, this is the text as it existed at that time.
METHOD FOR CONSTRUCTING SINGLE CELL LIBRARY CONTAINING TISSUE LOCATION INFORMATION AND SEQUENCING METHOD
Application US20260109969A1 Kind: A1 Apr 23, 2026
Assignee
BEIJING SEEKGENE BIOSCIENCES CO., LTD.
Inventors
Jinhuan Han, Ying Guan, Xiaoling Zhi, Min Zhi, Guoqin Sang, Huanhuan Shi, Shaozhuo Jiao, Zongwen Li
Abstract
Disclosed in the present invention are a method for constructing a single cell library containing tissue location information and a sequencing method. The construction method comprises the following steps: S10, providing a chip, wherein the chip is provided with a plurality of sites, each site is provided with nucleic acid molecules having a known base sequence, and the nucleic acid molecules contain location information; S20, enabling a tissue slice to be tested to be in contact with the chip, so as to bind the nucleic acid molecules having the known base sequence in the chip to cells of said tissue slice to obtain a bound tissue slice; S30, dissociating the bound tissue slice to prepare a single cell suspension; S40, constructing a library for the single cell suspension by means of a high-throughput single cell library construction and sequencing platform, to obtain a transcriptome library and a spatial barcoded library; and S50, sequencing the transcriptome library and the spatial barcoded library to obtain a single cell library containing tissue location information. According to the method, spatial location information of a single cell can be tested while analyzing genetic information on a single cell level.
CPC Classifications
C12N 15/1065
Filing Date
2024-02-07
Application No.
19117937
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