Multi-Partite CRISPR Editors, High-Throughput Discovery Methods
Summary
US Patent Application US20260109972A1, filed November 9, 2023, has been published by the USPTO, disclosing multi-partite CRISPR-based editor compositions and high-throughput methods for discovering polypeptides with editing activity. Inventors include Luke A. Gilbert, Greg Pommier, Jonathan Weissman, and Caroline Wilson. The publication makes the application's technical disclosure publicly accessible as prior art.
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GovPing monitors USPTO Patent Applications - Biotech (C12N) for new healthcare & life sciences regulatory changes. Every update since tracking began is archived, classified, and available as free RSS or email alerts — 264 changes logged to date.
What changed
The USPTO has published patent application US20260109972A1, which discloses methods for determining whether candidate polypeptides in a library exhibit editing activity using multi-partite CRISPR-based editors. The disclosed methods involve expressing in cells a library of expression cassettes encoding polypeptides that include a first editing domain, a nuclease-deficient RNA-guided endonuclease domain, and a second editing domain, together with a guide RNA expression cassette targeting a sequence of interest.
Affected parties such as patent applicants, biotechnology researchers, and genome editing companies should monitor this publication as prior art that may affect freedom-to-operate analyses and competitive patent landscapes in the CRISPR gene editing space. No compliance obligations arise solely from the publication of a patent application.
Archived snapshot
Apr 23, 2026GovPing captured this document from the original source. If the source has since changed or been removed, this is the text as it existed at that time.
High-Throughput Discovery of Multi-Partite CRISPR-Based Editors
Application US20260109972A1 Kind: A1 Apr 23, 2026
Inventors
Luke A. Gilbert, Greg Pommier, Jonathan Weissman, Caroline Wilson
Abstract
Provided are methods for determining that a candidate polypeptide in a library of polypeptides has editing activity comprising expressing in a plurality of cells: a library of first expression cassettes each comprising a nucleic acid encoding a polypeptide wherein each polypeptide in the library comprises, a first editing domain, a first linker, a nuclease-deficient RNA guided endonuclease domain, a second linker, and a second editing domain; and a second expression cassette comprising a nucleic acid encoding a guide RNA directed to a target of interest, wherein said first and second expression cassettes are expressed in the plurality of cells; separating the plurality of cells based on the expression level of the target of interest; extracting genomic DNA (gDNA) from the plurality of cells; and sequencing the gDNA of cells that have a high level expression or a low level of expression of the target of interest.
CPC Classifications
C12N 15/1082
Filing Date
2023-11-09
Application No.
19127007
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