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Recombinant Vaccine Against Helminths in Pichia pastoris

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Summary

USPTO published patent application US20260092089A1 by Fundação Oswaldo Cruz disclosing recombinant production of Sm14 Schistosoma mansoni vaccine antigen using genetically engineered Pichia pastoris strains with methanol-inducible (AOX1) and constitutive (GAP) promoters. The application covers synthetic gene construction, fermentation processes, and protein purification methods scalable for industrial vaccine manufacturing.

What changed

The USPTO published a patent application (US20260092089A1) by Fundação Oswaldo Cruz for a recombinant vaccine against helminths using Pichia pastoris yeast as expression host. The invention describes creation of a synthetic gene optimized for high expression of Sm14 Schistosoma mansoni protein, cloned under two promoter systems (AOX1 methanol-inducible and GAP constitutive) for efficient production of the vaccine antigen. The application covers the genetic constructs, fermentation methods, and purification processes for industrial-scale manufacturing.

This is a patent application publication only—no compliance obligations or deadlines apply. Parties interested in this technology should monitor application status for examination results. Researchers and vaccine developers may explore potential licensing or partnership opportunities with Fundação Oswaldo Cruz if the patent proceeds to grant. Commercial development would require separate regulatory approvals for any resulting vaccine product.

Archived snapshot

Apr 2, 2026

GovPing captured this document from the original source. If the source has since changed or been removed, this is the text as it existed at that time.

← USPTO Patent Applications

RECOMBINANT VACCINE AGAINST HELMINTHS IN PICHIA PASTORIS AND METHODS FOR PRODUCING AND PURIFYING PROTEINS FOR USE AS VACCINES AGAINST HELMINTHS

Application US20260092089A1 Kind: A1 Apr 02, 2026

Assignee

Fundação Oswaldo Cruz

Inventors

Miriam TENDLER, Andrew J. SIMPSON, Celso Raul Romero RAMOS

Abstract

The present invention is related to the recombinant production of proteins by using a synthetic gene for high protein expression in Pichia pastoris. More specifically, the invention describes the production of Sm14 Schistosoma mansoni recombinant protein, where a synthetic gene was created to promote high expression of such protein, a gene which was cloned under control of two types of Pichia pastoris promoters: methanol-inducible promoter (AOXI) and constituent promoter (GAP). With these constructions, Pichia pastoris strains were genetically manipulated to efficiently produce vaccine antigen Sm14. The processes to produce and purify this protein from P. pastoris cells, which can be escalated for their industrial production, were also improved.

CPC Classifications

C07K 14/43559 A61K 39/0003 C12N 15/815

Filing Date

2025-11-28

Application No.

19403587

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Last updated

Classification

Agency
USPTO
Published
April 2nd, 2026
Instrument
Notice
Legal weight
Non-binding
Stage
Draft
Change scope
Minor
Document ID
US20260092089A1

Who this affects

Applies to
Pharmaceutical companies Drug manufacturers Healthcare providers
Industry sector
3254.1 Biotechnology 3254 Pharmaceutical Manufacturing
Activity scope
Patent Filings Biotechnology Research
Geographic scope
United States US

Taxonomy

Primary area
Intellectual Property
Operational domain
Legal
Topics
Healthcare Pharmaceuticals Biotechnology

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