Methods for adding polymers to ribonucleic acids
Summary
The USPTO has published a patent application detailing methods for adding polymers of non-canonical nucleotides to the 3' end of ribonucleic acids (RNA). The application, filed by inventors from institutions including the University of California, describes techniques that may be used for RNA analysis and identification. This publication represents a new patent application and does not impose immediate regulatory obligations.
What changed
The United States Patent and Trademark Office (USPTO) has published patent application US20260085305A1, titled "Methods for adding polymers to ribonucleic acids." This application, filed on May 20, 2025, by inventors Manuel Ares, Jr., Logan Mulroney, Jenny Vo, and Mark Akeson, describes methods for attaching polymers of non-canonical nucleotides to the 3' end of RNA using specific enzymes. The described techniques may also facilitate RNA analysis, including identifying the polymer and determining the junction point with the RNA.
This publication is a patent application and does not constitute a rule or regulation with immediate compliance requirements. However, it signals potential future developments in biotechnology and RNA modification. Companies involved in RNA research, development, or therapeutics should monitor this patent application and related intellectual property developments. The application is classified under CPC codes related to biotechnology and enzyme activity, indicating its relevance to the pharmaceutical and biotechnology sectors.
Archived snapshot
Mar 27, 2026GovPing captured this document from the original source. If the source has since changed or been removed, this is the text as it existed at that time.
METHODS OF ADDING POLYMERS TO RIBONUCLEIC ACIDS
Application US20260085305A1 Kind: A1 Mar 26, 2026
Inventors
Manuel Ares, Jr., Logan Mulroney, Jenny Vo, Mark Akeson
Abstract
Provided are methods of adding a polymer of non-canonical nucleotides to the 3′ end of a ribonucleic acid (RNA). In certain embodiments, the methods comprise combining an RNA, a polynucleotide-3′ nucleotidyl transferase, and non-canonical nucleotides, in a reaction mixture under conditions in which the polynucleotide-3′ nucleotidyl transferase adds a polymer of the non-canonical nucleotides to the 3′ end of the RNA. Such methods may further include analyzing the RNA using a nanopore. According to some embodiments, the methods include identifying the polymer of non-canonical nucleotides added to the 3′ end of the RNA, and determining the junction between 3′ end of the RNA and the polymer of non-canonical nucleotides to identify 3′0 end of the RNA. Kits that find use, e.g., in practicing the methods of the present disclosure are also provided.
CPC Classifications
C12N 15/102 C12N 9/1282 C12Y 207/07052 C12Y 207/07072
Filing Date
2025-05-20
Application No.
19213794
Related changes
Get daily alerts for USPTO Patent Applications - Biotech (C12N)
Daily digest delivered to your inbox.
Free. Unsubscribe anytime.
Source
About this page
Every important government, regulator, and court update from around the world. One place. Real-time. Free. Our mission
Source document text, dates, docket IDs, and authority are extracted directly from USPTO.
The summary, classification, recommended actions, deadlines, and penalty information are AI-generated from the original text and may contain errors. Always verify against the source document.
Classification
Who this affects
Taxonomy
Browse Categories
Get alerts for this source
We'll email you when USPTO Patent Applications - Biotech (C12N) publishes new changes.
Subscribed!
Optional. Filters your digest to exactly the updates that matter to you.