Element Biosciences PCR-Free Library Preparation Patent Granted
Summary
The USPTO granted US Patent 12,606,819B2 to Element Biosciences, Inc. covering compositions and methods for PCR-free library preparation using double-stranded splint adaptors. The patent claims double-stranded splint adaptor structures with long and short hybridized strands carrying adaptor sequences, as well as one-pot multi-enzyme reaction workflows for introducing new adaptor sequences into library molecules. The patent contains 30 claims across CPC classifications including C12N 15/1065 and C12Q 1/6855.
What changed
The USPTO issued Patent 12,606,819B2 to Element Biosciences, Inc., covering compositions comprising nucleic acid double-stranded splint adaptors and methods employing them in PCR-free workflows. The double-stranded splint adaptors comprise first and second splint strands hybridized together with a double-stranded region and two flanking single-stranded regions, where the second strand carries adaptor sequences such as universal binding sequences and/or index sequences.
For firms developing nucleic acid library preparation kits or sequencing workflows, this patent establishes IP protection around specific splint-based adaptor designs and one-pot multi-enzyme reaction methods. Competitors in the genomics or next-generation sequencing space should review their library preparation approaches to assess potential overlap with the claimed adaptor structures and PCR-free workflows.
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Apr 21, 2026GovPing captured this document from the original source. If the source has since changed or been removed, this is the text as it existed at that time.
PCR-free library preparation using double-stranded splint adaptors and methods of use
Grant US12606819B2 Kind: B2 Apr 21, 2026
Assignee
Element Biosciences, Inc.
Inventors
Junhua Zhao, Xiaodong Qi, Shawn Levy
Abstract
The present disclosure provides compositions comprising nucleic acid double-stranded splint adaptors, including kits, and methods that employ the double-stranded splint adaptors, e.g., PCR-free workflows. The double-stranded splint adaptors (200) can be used in a one-pot, multi-enzyme reaction to introduce one or more new adaptor sequences into a library molecule. The double-stranded splint adaptor (200) comprises a first splint strand (long splint strand (300)) and a second splint strand (short splint strand (400)), where the first and second splint strands are hybridized together to form the double-stranded splint adaptor (200) having a double-stranded region and two flanking single-stranded regions. The second splint strand (400) carries the new adaptor sequence(s) to be introduced, such as for example a universal binding sequence and/or an index sequence.
CPC Classifications
C12N 15/1065 C12Q 1/6855 C12Q 2525/155 C12Q 2525/191 C12Q 2531/125 C12Q 2535/122
Filing Date
2023-07-05
Application No.
18347440
Claims
30
Mentioned entities
Parties
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