Self-Circularized RNA Preparation Method, Apr 09

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METHOD OF PREPARING SELF-CIRCULARIZED RNA

Application US20260098261A1 Kind: A1 Apr 09, 2026

Inventors

FENGMEI PI, JARRED RENSVOLD, CHENG-HSIEN WU

Abstract

A method of preparing a circular RNA includes transcribing a vector to form a precursor RNA, in which the vector includes the following elements operably connected to each other and arranged in the following sequence: a) a 5′ element, b) a 3′ Group I self-splicing intron fragment containing a 3′ splice site dinucleotide, c) none or an element containing an internal ribosome entry site (IRES) and a protein coding region or an element containing a noncoding region, d) a 5′ Group I self-splicing intron fragment containing a 5′ splice site dinucleotide, and e) a 3′ element, in which 5′ element and 3′ element form a stable structure with a Gibbs free energy (ΔG) from −190 kcal/mol to −9.0 kcal/mol, provided that the stable structure is not a duplex with at least 95% base pairing between 5′ element and 3′ element, in which 3′ Group I self-splicing intron fragment and 5′ Group I self-splicing intron fragment form a self-cleaving and self-ligating RNA molecule, thereby generating circular RNA.

CPC Classifications

C12N 15/111 C12N 15/66 C12N 15/85 C12N 2310/532 C12N 2840/203

Filing Date

2025-09-19

Application No.

19334099

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