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Protein Evolution System With DNA Synthesis, Microfluidics

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Summary

The USPTO published patent application US20260098257A1 covering systems, methods, and kits for rapid protein evolution. The application (No. 19271120, filed July 16, 2025) names five inventors and describes a system combining DNA synthesis, microfluidic devices with sequestration pens, and a computing component for iterative protein optimization. The technology enables cell-free protein expression and assay-based evaluation of protein variants.

What changed

The USPTO published a patent application (US20260098257A1) for a protein evolution system incorporating DNA synthesis, a microfluidic device with sequestration pens, and a computing component for assay analysis and improved DNA sequence design. The system enables correlation of DNA sequence location on a bead within the microfluidic device, cell-free protein expression, and protein assay evaluation. Application No. 19271120 was filed July 16, 2025 and published April 9, 2026.

Manufacturers and researchers in biotechnology and pharmaceutical development should monitor this application as it may indicate направление в области protein engineering tools. Patent applications do not yet confer enforceable IP rights; they signal potential future licensing or freedom-to-operate considerations.

What to do next

  1. Monitor for patent grant or office action

Archived snapshot

Apr 13, 2026

GovPing captured this document from the original source. If the source has since changed or been removed, this is the text as it existed at that time.

← USPTO Patent Applications

SYSTEMS AND METHODS FOR IDENTIFICATION OF OPTIMIZED PROTEIN PRODUCTION AND KITS THEREFOR

Application US20260098257A1 Kind: A1 Apr 09, 2026

Inventors

Jason M. MCEWEN, Troy A. LIONBERGER, Eric K. SACKMANN, Volker L.S. KURZ, Kellen C. MOBILIA

Abstract

Systems, methods, and kits therefor, enabling rapid protein evolution are described herein. A system useful in the methods described herein include a DNA synthesis component; a microfluidic system including a microfluidic device having a microfluidic channel and sequestration pens; and a computing component, which is configured to analyze assay results and, based upon the analysis, design improved DNA sequences for iterative protein evolution. The microfluidic system is configured to permit correlation of DNA sequence on a bead to its location within the microfluidic device, permit cell free protein expression of a DNA sequence captured to a bead, and to permit assay of the protein so produced.

CPC Classifications

C12N 15/1075 B01L 3/502761 C12N 15/1065 B01L 2200/0647

Filing Date

2025-07-16

Application No.

19271120

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Last updated

Classification

Agency
USPTO
Published
April 9th, 2026
Instrument
Notice
Legal weight
Non-binding
Stage
Final
Change scope
Minor
Document ID
US20260098257A1

Who this affects

Applies to
Manufacturers
Industry sector
3254.1 Biotechnology
Activity scope
Patent examination IP licensing
Geographic scope
United States US

Taxonomy

Primary area
Intellectual Property
Operational domain
Legal
Topics
Pharmaceuticals Medical Devices

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