Protein Crystallization Method, Multiple Protein Feeds

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CRYSTALLISATION OF A PROTEIN CAPABLE OF CRYSTALLIZING UNDER SALTING-IN CONDITIONS USING MULTIPLE PROTEIN FEEDS

Application US20260098057A1 Kind: A1 Apr 09, 2026

Inventors

Hans BERTELSEN, Kasper BØGELUND LAURIDSEN, Nikolaj DRACHMANN

Abstract

The present invention relates to an improved method of producing products comprising a target protein (TP) capable of crystallising under salting-in conditions by crystallisation of the TP. The invention makes use of at least two different TP-containing protein feeds which are mixed to prepare a supersaturated protein solution in which TP is crystallized. One of the protein feeds must have a pH of at least the pH providing the maximum crystallisation yield of the TP (pHMCY,TP) plus 0.1 and one of the protein feeds must have a pH of at most pHMCY,TP minus 0.1.

CPC Classifications

C07K 1/306 C07K 14/4717 C07K 14/555 C07K 14/605 C07K 14/62 C07K 16/11 C07K 16/18 C07K 16/241 C07K 16/2818 C07K 16/2827 C07K 16/2863 C07K 16/2866 C07K 16/2875 C07K 16/2887 C07K 16/32 C12N 9/0006 C12N 9/0061 C12N 9/0065 C12N 9/16 C12N 9/20 C12N 9/2402 C12N 9/2414 C12N 9/2428 C12N 9/2437 C12N 9/2457 C12N 9/2462 C12N 9/2471 C12N 9/50 C12N 9/88 C12N 9/90 C12Y 101/03004 C12Y 110/03002 C12Y 111/01014 C12Y 301/01003 C12Y 302/01001 C12Y 302/01003 C12Y 302/01004 C12Y 302/01008 C12Y 302/01011 C12Y 302/01017 C12Y 302/0102 C12Y 302/01023 C12Y 302/01041 C12Y 302/01067 C12Y 302/01108 C12Y 304/21001 C12Y 304/23004

Filing Date

2022-08-10

Application No.

19099236

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