USPTO Patent Grant: DNA Fragment Assembly Method and Kit
Summary
The USPTO has granted patent US12584155B2 for a method and kit for assembling multiple DNA fragments at room temperature. The patent, assigned to Universitetet i Tromsø—Norges arktiske universitet, utilizes specific thermolabile DNA polymerases and exonucleases.
What changed
The United States Patent and Trademark Office (USPTO) has issued patent US12584155B2, titled 'Method and kit for assembly of multiple DNA fragments at room temperature.' This patent, assigned to Universitetet i Tromsø—Norges arktiske universitet, covers a novel method for assembling DNA fragments using specific thermolabile DNA polymerases and exonucleases derived from marine organisms. The patent was filed on October 1, 2020, and granted on March 24, 2026, with 13 claims.
This patent grant is primarily an intellectual property matter and does not impose direct compliance obligations on regulated entities. However, companies involved in DNA synthesis, genetic engineering, or biotechnology research may need to be aware of this patent to ensure they are not infringing on the intellectual property rights of the assignee. Companies may wish to review their internal processes and product development pipelines to assess potential conflicts and consider licensing or other arrangements if their activities overlap with the patented method.
Source document (simplified)
Method and kit for assembly of multiple DNA fragments at room temperature
Grant US12584155B2 Kind: B2 Mar 24, 2026
Assignee
UNIVERSITETET I TROMSØ—NORGES ARKTISKE UNIVERSITET
Inventors
Yvonne Piotrowski, Atle Noralf Larsen
Abstract
The present invention relates to recombinant DNA technology, in particular to methods for assembling two or more double stranded (ds) nucleic acid molecules with overlapping terminal sequences. In particular, the present invention relates to the use of a thermolabile DNA polymerase II derived 3′-5′ exonuclease isolated from Moritella viscoa and a thermolabile DNA polymerase I of marine origin in multi DNA assembly processes.
CPC Classifications
C12N 9/1252 C12Y 207/07007
Filing Date
2020-10-01
Application No.
17765710
Claims
13
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