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USPTO Patent Grant: DNA Fragment Assembly Method and Kit

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Published March 24th, 2026
Detected March 24th, 2026
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Summary

The USPTO has granted patent US12584155B2 for a method and kit for assembling multiple DNA fragments at room temperature. The patent, assigned to Universitetet i Tromsø—Norges arktiske universitet, utilizes specific thermolabile DNA polymerases and exonucleases.

What changed

The United States Patent and Trademark Office (USPTO) has issued patent US12584155B2, titled 'Method and kit for assembly of multiple DNA fragments at room temperature.' This patent, assigned to Universitetet i Tromsø—Norges arktiske universitet, covers a novel method for assembling DNA fragments using specific thermolabile DNA polymerases and exonucleases derived from marine organisms. The patent was filed on October 1, 2020, and granted on March 24, 2026, with 13 claims.

This patent grant is primarily an intellectual property matter and does not impose direct compliance obligations on regulated entities. However, companies involved in DNA synthesis, genetic engineering, or biotechnology research may need to be aware of this patent to ensure they are not infringing on the intellectual property rights of the assignee. Companies may wish to review their internal processes and product development pipelines to assess potential conflicts and consider licensing or other arrangements if their activities overlap with the patented method.

Source document (simplified)

← USPTO Patent Grants

Method and kit for assembly of multiple DNA fragments at room temperature

Grant US12584155B2 Kind: B2 Mar 24, 2026

Assignee

UNIVERSITETET I TROMSØ—NORGES ARKTISKE UNIVERSITET

Inventors

Yvonne Piotrowski, Atle Noralf Larsen

Abstract

The present invention relates to recombinant DNA technology, in particular to methods for assembling two or more double stranded (ds) nucleic acid molecules with overlapping terminal sequences. In particular, the present invention relates to the use of a thermolabile DNA polymerase II derived 3′-5′ exonuclease isolated from Moritella viscoa and a thermolabile DNA polymerase I of marine origin in multi DNA assembly processes.

CPC Classifications

C12N 9/1252 C12Y 207/07007

Filing Date

2020-10-01

Application No.

17765710

Claims

13

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Source

Analysis generated by AI. Source diff and links are from the original.

Classification

Agency
USPTO
Published
March 24th, 2026
Instrument
Notice
Legal weight
Non-binding
Stage
Final
Change scope
Minor
Document ID
US12584155B2

Who this affects

Applies to
Drug manufacturers Medical device makers Pharmaceutical companies
Industry sector
3254 Pharmaceutical Manufacturing 3254.1 Biotechnology
Activity scope
DNA Synthesis Genetic Engineering
Geographic scope
United States US

Taxonomy

Primary area
Pharmaceuticals
Operational domain
Research & Development
Topics
Biotechnology Intellectual Property

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