Method and kit for assembly of multiple DNA fragments at room temperature
Grant
US12584155B2
Kind: B2
Mar 24, 2026
Assignee
UNIVERSITETET I TROMSØ—NORGES ARKTISKE UNIVERSITET
Inventors
Yvonne Piotrowski, Atle Noralf Larsen
Abstract
The present invention relates to recombinant DNA technology, in particular to methods for assembling two or more double stranded (ds) nucleic acid molecules with overlapping terminal sequences. In particular, the present invention relates to the use of a thermolabile DNA polymerase II derived 3′-5′ exonuclease isolated from Moritella viscoa and a thermolabile DNA polymerase I of marine origin in multi DNA assembly processes.
CPC Classifications
C12N 9/1252
C12Y 207/07007
Filing Date
2020-10-01
Application No.
17765710
Claims
13