Double Tagged Serratia Marcescens Nuclease Patent Application

ChangeBridge: Patent Apps - Biotech (C12N)

Published April 2nd, 2026

Detected April 2nd, 2026

Summary

USPTO published patent application US20260092265A1 for a double-tagged Serratia Marcescens Nuclease (SMN) engineered with a C-terminal Chitin Binding Domain (CBD-tag) and a 6-mer poly-histidine tag connected by a Gly-Ser linker. Inventors: Dapeng Sun, Aine Quimby, Zhenyu Zhu. Application No. 19324955 was filed September 10, 2025.

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What changed

USPTO published a patent application describing a recombinant Serratia Marcescens Nuclease (SMN) protein engineered to include two affinity tags at its C-terminus: a Chitin Binding Domain (CBD-tag) followed by a 6-mer poly-histidine tag separated by a Gly-Ser linker. The dual-tag configuration enables the enzyme to be easily removed from reaction solutions following nucleic acid digestion or bound to solid supports for sample processing. CPC classifications include C12N 9/16, C08B 37/003, and C12Y 301/30002, indicating applications in enzyme technology and carbohydrate biochemistry.

Patent applications do not impose compliance obligations on third parties. Companies engaged in nucleic acid digestion, enzyme purification, or recombinant protein manufacturing should monitor this application's prosecution to assess potential freedom-to-operate implications for related technologies. No action is required at this stage beyond routine IP portfolio review.

Source document (simplified)

← USPTO Patent Applications

Double Tagged Serratia Marcescens Nuclease

Application US20260092265A1 Kind: A1 Apr 02, 2026

Inventors

Dapeng Sun, Aine Quimby, Zhenyu Zhu

Abstract

Either wild type or mutant Serratia Marcescens Nuclease (“SMN”) is engineered to display a C-terminal Chitin Binding Domain (CBD-tag), followed, at the C-terminus side of the CBD-tag, by a poly-histidine tag (His-tag), where the His-tag is preferably a 6-mer and preferably is preceded by a Gly-Ser linker, thereby generating a recombinant SMN protein that retains dual affinity tags (a CBD-tag and a His-tag) at the C-terminus, to make it easily removed from a reaction solution following digestion of nucleic acids in the reaction mixture. It can also be used for binding SMN to a solid support for use in nucleic acid digestion in a sample contacted with the solid support.