DNA Library Generation Patent - Menarini Silicon Biosystems
Summary
The USPTO granted Patent US12601010B2 to Menarini Silicon Biosystems S.p.A. for a method and kit generating DNA libraries for massively parallel sequencing. The patent covers a four-step process involving WGA DNA library preparation, two single-cycle PCR reactions using specific primer sequences with sequencing adapters, and a final amplification step. The patent includes 8 claims and was filed November 10, 2023 under application number 18506751.
What changed
The USPTO issued Patent US12601010B2 to Menarini Silicon Biosystems S.p.A. covering methods and kits for generating DNA libraries used in massively parallel sequencing. The patent describes a primary WGA DNA library preparation followed by two single-cycle PCR amplifications using primers containing distinct sequencing adapters, with a final PCR amplification step. The CPC classifications include C12Q 1/6874 (measuring/Investigating nucleic acid processes/combinations), C12Q 2600/16 (nucleic acid measurement data), and C12N 15/1093 (nucleic acid processes/techniques for cloning/expression).
For companies developing or using next-generation sequencing library preparation methods, this patent establishes exclusive rights for Menarini Silicon Biosystems in the US market. Competitors in the genomic sequencing space should conduct freedom-to-operate analyses before commercializing similar library preparation methods. Research institutions and clinical laboratories using these techniques may need to evaluate licensing requirements. The patent's scope covers both the method steps and associated kit compositions for DNA library generation.
What to do next
- Monitor patent claims for freedom-to-operate analysis
- Evaluate licensing options for commercially implementing the patented method
- Review patent for potential relevance to existing sequencing workflows
Archived snapshot
Apr 16, 2026GovPing captured this document from the original source. If the source has since changed or been removed, this is the text as it existed at that time.
Method and kit for the generation of DNA libraries for massively parallel sequencing
Grant US12601010B2 Kind: B2 Apr 14, 2026
Assignee
Menarini Silicon Biosystems S.p.A.
Inventors
Valentina Del Monaco, Nicolò Manaresi, Genny Buson, Paola Tononi
Abstract
There is disclosed a method of generating a massively parallel sequencing library comprising the steps of :a) providing a primary WGA DNA library (pWGAlib), including fragments comprising a WGA library universal sequence adapter; b) performing a single PCR cycle on the pWGAlib using a first primer (1PR) comprising from 5′ to 3′ a first sequencing adapter (1PR5SA) and a first primer 3′ section (1PR3S) hybridizing to the reverse complementary of the WGA library universal sequence adapter; c) performing a single PCR cycle on the on the product of step b) using a second primer (2PR) comprising from 5′ to 3′ a second sequencing adapter (2PR5SA) different from the 1PR5SA, and a second primer 3′ section (2PR3S) hybridizing to the WGA library universal sequence adapter reverse complementary; d) amplifying by PCR the product of step c) using a third primer comprising the 1PR5SA and a fourth primer comprising 2PR5SA.
CPC Classifications
C12Q 1/6874 C12Q 2600/16 C12N 15/1093
Filing Date
2023-11-10
Application No.
18506751
Claims
8
Named provisions
Related changes
Get daily alerts for USPTO Patent Grants - Biotech (C12N)
Daily digest delivered to your inbox.
Free. Unsubscribe anytime.
Source
About this page
Every important government, regulator, and court update from around the world. One place. Real-time. Free. Our mission
Source document text, dates, docket IDs, and authority are extracted directly from USPTO.
The summary, classification, recommended actions, deadlines, and penalty information are AI-generated from the original text and may contain errors. Always verify against the source document.
Classification
Who this affects
Taxonomy
Browse Categories
Get alerts for this source
We'll email you when USPTO Patent Grants - Biotech (C12N) publishes new changes.
Subscribed!
Optional. Filters your digest to exactly the updates that matter to you.