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Transgenic Non-Human Animals Producing Modified Heavy Chain-Only Antibodies

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Summary

USPTO published patent application US20260090529A1 disclosing transgenic non-human animals that produce modified heavy chain-only antibodies (HCAbs). The invention involves substituting the native amino acid residue at the first position of framework region FR4 with a different residue to disrupt a surface-exposed hydrophobic patch, potentially improving antibody characteristics. Named inventors include Wim van Schooten, Nathan Trinklein, Shelley Force Aldred, Marianne Bruggemann, and Mike Osborn.

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What changed

USPTO published patent application US20260090529A1 for transgenic non-human animals producing modified heavy chain-only antibodies. The application claims animals genetically modified to produce HCAbs where the native amino acid at position one of FR4 is substituted with a different residue capable of disrupting a surface-exposed hydrophobic patch. The application (No. 19393163) was filed November 18, 2025, and published April 2, 2026, covering CPC classifications spanning A01K (transgenic animals) and C07K (peptides/proteins including antibody frameworks C07K 2317/21 through C07K 2317/569).

This patent application does not impose compliance obligations on regulated entities. Biotechnology and pharmaceutical companies developing antibody therapeutics should monitor this application to assess potential licensing implications or freedom-to-operate considerations for similar HCAb engineering approaches. Researchers and developers working with heavy chain-only antibody platforms should review the specific FR4 modifications claimed to understand the scope of protected modifications.

Archived snapshot

Apr 2, 2026

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← USPTO Patent Applications

TRANSGENIC NON-HUMAN ANIMALS PRODUCING MODIFIED HEAVY CHAIN-ONLY ANTIBODIES

Application US20260090529A1 Kind: A1 Apr 02, 2026

Inventors

Wim van Schooten, Nathan Trinklein, Shelley Force Aldred, Marianne Bruggemann, Mike Osborn

Abstract

Human or chimeric heavy chain-only antibodies are provided, in the native amino acid residue at the first position of the fourth framework region (FR4) of said HCAb is substituted by a different amino acid residue that is capable of disrupting a surface-exposed hydrophobic patch comprising or associated with the native amino acid residue at that position.

CPC Classifications

A01K 67/0275 A01K 67/027 C07K 16/00 A01K 2217/052 A01K 2227/105 A01K 2267/01 C07K 2317/21 C07K 2317/24 C07K 2317/52 C07K 2317/522 C07K 2317/565 C07K 2317/567 C07K 2317/569 C12N 15/902

Filing Date

2025-11-18

Application No.

19393163

View original document →

Named provisions

Modified Heavy Chain-Only Antibodies Transgenic Non-Human Animals Amino Acid Substitution at FR4 Position 1 Surface-Exposed Hydrophobic Patch Disruption

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Last updated

Classification

Agency
USPTO
Published
April 2nd, 2026
Instrument
Notice
Legal weight
Non-binding
Stage
Final
Change scope
Minor
Document ID
US20260090529A1

Who this affects

Applies to
Drug manufacturers Healthcare providers Research institutions
Industry sector
3254 Pharmaceutical Manufacturing 3254.1 Biotechnology 6211 Healthcare Providers
Activity scope
Patent Filings Biotechnology Research Antibody Development
Geographic scope
United States US

Taxonomy

Primary area
Intellectual Property
Operational domain
Legal
Topics
Healthcare Pharmaceuticals

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