Phenotypic and Genotypic Detection of Biofilm Formation and Efflux Pump Activity in Multi-drug Resistant Klebsiella Pneumoniae
Summary
NIH's ClinicalTrials.gov registered observational study NCT07541690, an examination of phenotypic and genotypic mechanisms of antibiotic resistance in multi-drug resistant Klebsiella Pneumoniae. The study will analyze 70 urine, pus, and sputum samples using culture, biochemical testing, antimicrobial susceptibility profiling, and PCR-based gene detection for biofilm formation and efflux pump activity. No compliance obligations are created by this registry entry.
“Antimicrobial susceptibility testing of the isolates will be performed using the Kirby-Bauer method (disc diffusion method) using Muller Hinton agar according to the Clinical and Laboratory Standards Institute (CLSI 2025) guidelines for selected groups of antibiotics.”
What changed
This document is a clinical trial registry entry for study NCT07541690, which will examine phenotypic and genotypic mechanisms underlying multi-drug resistance in Klebsiella Pneumoniae. The study protocol involves culture-based identification, antimicrobial susceptibility testing per CLSI 2025 guidelines, phenotypic detection of biofilm and efflux pump activity, and conventional PCR detection of specific resistance genes. Registry entries on ClinicalTrials.gov do not create regulatory obligations for healthcare providers, laboratories, or pharmaceutical companies. Researchers studying similar antimicrobial resistance mechanisms may find this protocol relevant to their own study design.
Affected parties include clinical microbiology researchers, infectious disease specialists, and antimicrobial resistance surveillance programs. The study does not impose reporting requirements, testing mandates, or compliance deadlines on external entities. No penalties or enforcement mechanisms are associated with this registry entry.
Archived snapshot
Apr 22, 2026GovPing captured this document from the original source. If the source has since changed or been removed, this is the text as it existed at that time.
Phenotypic and Genotypic Detection of Biofilm Formation and Efflux Pump Activity in Multi-drug Resistant Klebsiella Pneumoniae
Observational NCT07541690 Kind: OBSERVATIONAL Apr 21, 2026
Abstract
- Sample: urine, pus and sputum samples.
- Culture: the samples will be inoculated on the MacConkey agar and subcultured on eosine methylene blue agar.
- Identification of isolates will be done by:
- Colony morphology.
- Gram staining.
- Biochemical reactions: including Indole, Methyl red, Voges-Proskauer, and Citrate utilization (IMViC) tests.
- Antimicrobial susceptibility testing of the isolates will be performed using the Kirby-Bauer method (disc diffusion method) using Muller Hinton agar according to the Clinical and Laboratory Standards Institute (CLSI 2025) guidelines for selected groups of antibiotics.
- Phenotypic detection of biofilm formation by the microtitre plate method.
- Phenotypic detection of efflux pump activity by ethidium bromide-agar cartwheel method.
- Detection of biofilm genes (fimH-1, mrkA, and mrkD) by conventional PCR.
- Detection of efflux pump genes (acrAB, tolC, and mdtk) by conventional PCR.
Conditions: Multidrug Resistant Klebsiella Pneumoniae Infection
Interventions: Genotypic Detection of Biofilm Formation and Efflux Pump Activity.
Related changes
Get daily alerts for ClinicalTrials.gov Studies
Daily digest delivered to your inbox.
Free. Unsubscribe anytime.
Source
About this page
Every important government, regulator, and court update from around the world. One place. Real-time. Free. Our mission
Source document text, dates, docket IDs, and authority are extracted directly from NIH.
The summary, classification, recommended actions, deadlines, and penalty information are AI-generated from the original text and may contain errors. Always verify against the source document.
Classification
Who this affects
Taxonomy
Browse Categories
Get alerts for this source
We'll email you when ClinicalTrials.gov Studies publishes new changes.
Subscribed!
Optional. Filters your digest to exactly the updates that matter to you.