Split PYP Complementation System for Detecting Protein Interactions
Summary
USPTO granted patent US12590127B2 to PARIS SCIENCES ET LETTRES for a split photoactive yellow protein (PYP) complementation system for detecting protein interactions. The patent covers two PYP fragments with at least 70% sequence identity to SEQ ID NO:23 and SEQ ID NO:34, used with fluorogenic HBR analogs for protein interaction detection. The grant includes 19 allowed claims.
What changed
USPTO issued Patent No. US12590127B2 to inventors Arnaud Gautier and Alison Tebo, assigned to PARIS SCIENCES ET LETTRES, covering a split photoactive yellow protein (PYP) complementation system. The invention comprises two PYP fragments—one with at least 70% identity to SEQ ID NO:23 (including truncated fragments of at least 89 consecutive C-terminal amino acids) and another with at least 70% identity to SEQ ID NO:34 (including truncated fragments of at least 8 consecutive N-terminal amino acids)—used with hydroxybenzylidene rhodanine analogs for detecting protein-protein interactions.
This patent grant establishes exclusive rights for the assignee in the United States. Entities conducting protein interaction research or developing protein detection assays should review the patent claims to assess potential licensing needs. The patent is classified under CPC codes C07K 14/195, C07K 7/06, G01N 33/582, and related categories, indicating broad applicability in biotechnology and pharmaceutical research contexts.
Archived snapshot
Mar 31, 2026GovPing captured this document from the original source. If the source has since changed or been removed, this is the text as it existed at that time.
Split photoactive yellow protein complementation system and uses thereof
Grant US12590127B2 Kind: B2 Mar 31, 2026
Assignee
PARIS SCIENCES ET LETTRES
Inventors
Arnaud Gautier, Alison Tebo
Abstract
A complementation system including two fragments of photoactive yellow protein (PYP), or truncated fragments thereof, and its use with a fluorogenic hydroxybenzylidene rhodanine (HBR) analog for detecting interactions between biological molecules of interest, in particular between proteins of interest. Especially, a complementation system including a first PYP fragment having an amino acid sequence having at least about 70% identity with the amino acid sequence of SEQ ID NO: 23, or a truncated fragment thereof including at least 89 consecutive amino acids from the C-terminal end of the amino acid sequence; and a second PYP fragment having an amino acid sequence having at least about 70% identity with the amino acid sequence of SEQ ID NO: 34, or a truncated fragment thereof including at least 8 consecutive amino acids of the amino acid sequence, preferably 8 consecutive amino acids from the N-terminal end of the amino acid sequence.
CPC Classifications
C07K 14/195 C07K 7/06 C07K 19/00 C07K 2319/60 C07D 277/36 C07D 277/34 G01N 33/582 G01N 33/6845
Filing Date
2020-04-03
Application No.
17600329
Claims
19
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