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Use of ENTPD3 for identification, isolation, and enhancing mature stem cell derived insulin-producing cells

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Summary

The USPTO granted patent US12594306B2 to the Regents of the University of Colorado on April 7, 2026. The patent covers methods and compositions for identifying, sorting, and separating stem cell-derived pancreatic beta cells using the cell surface protein ENTPD3 (CD39L3) for diabetes treatment applications. The patent includes 13 claims and has a filing date of March 25, 2022.

What changed

The USPTO issued patent US12594306B2 to the University of Colorado covering methods for identifying, isolating, and enhancing mature stem cell-derived insulin-producing cells using ENTPD3 (CD39L3) for cell-based therapies targeting diabetes, including Type-1 diabetes. The patent discloses compositions and systems for live-sorting heterogeneous populations of stem cell-derived pancreatic beta cells into functionally homogeneous populations.\n\nResearch institutions, biotechnology companies, and pharmaceutical companies developing stem cell-based diabetes treatments should monitor this IP landscape. The patent may affect freedom-to-operate for competing approaches in cell therapy development targeting beta cell replacement or diabetes treatment. Licensing discussions may be warranted for parties developing similar cell sorting or identification methodologies.

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Archived snapshot

Apr 7, 2026

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← USPTO Patent Grants

Use of ENTPD3 for identification, isolation, and enhancing mature stem cell derived insulin-producing cells

Grant US12594306B2 Kind: B2 Apr 07, 2026

Assignee

The Regents of the University of Colorado, A Body Corporate

Inventors

Holger A. Russ, Fiona Docherty

Abstract

Disclosed herein are methods, systems, and compositions for enhancing the effectiveness of β-cell (Beta-cell)-based therapies. Also disclosed herein are methods, systems, and compositions related to identifying, sorting and separating heterogeneous populations of stem cell-derived pancreatic β-cells (sBCs) into more useful and functionally homogeneous cell populations. In many embodiments, the most mature and functional of the sBCs are identified and live-sorted using the cell surface protein Ectonucleoside Triphosphate Diphosphohydrolase-3 (ENTP3), which is also referred to as CD39L3. The presently disclosed methods, systems, and compositions are useful for cell therapies, for example replacement therapy. In many embodiments the disclosed systems, methods, and compositions are useful in treatments for diabetes. In some embodiments, the disclosed methods, systems, and compositions may be useful in treating, preventing, and/or curing diabetes, for example type-1 diabetes.

CPC Classifications

A61K 35/39

Filing Date

2022-03-25

Application No.

17704429

Claims

13

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Classification

Agency
USPTO
Published
April 7th, 2026
Instrument
Notice
Legal weight
Binding
Stage
Final
Change scope
Minor
Document ID
US12594306B2

Who this affects

Applies to
Educational institutions Biotechnology companies Pharmaceutical companies
Industry sector
3254 Pharmaceutical Manufacturing
Activity scope
Patent grants Biotechnology research Cell therapy development
Geographic scope
United States US

Taxonomy

Primary area
Intellectual Property
Operational domain
Legal
Topics
Healthcare Pharmaceuticals

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