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Cell-Free PPi-Driven ATP Regeneration Platform Patent

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Cell-Free PPi-Driven ATP Regeneration Platform Patent

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Apr 13, 2026

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CELL-FREE PPi-DRIVEN ATP REGENERATION PLATFORM

Application US20260098284A1 Kind: A1 Apr 09, 2026

Inventors

Stepan Tymoshenko

Abstract

Bio-production processes that rely on biological activity can be very slow and inefficient, yet, the output of these processes may have significant value. Using biological enzymes outside their natural cellular environments offers a significant and largely untapped opportunity to enhance bio-production processes. By decoupling enzymes from their native contexts, one can modify both their sequences and structures in ways that are favorable for industrial applications. This system and method mixes substrate molecules, PPi, and enzymes that utilize PPi (PPi-dependent enzymes) in a reaction chamber and ends with stable-form products, including phosphorylated molecules.

CPC Classifications

C12P 9/00 C12M 41/26 C12M 41/32 C12N 9/1229 C12N 9/1294 C12N 9/88 C12N 9/93 C12Y 207/04003 C12Y 207/09001 C12Y 401/01038 C12Y 402/01001 C12Y 604/01001

Filing Date

2025-10-03

Application No.

19349943

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