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Single-Chain Fragment Variable Targeting KRAS G12V CAR T-Cell Therapy

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Summary

USPTO published patent application US20260108609A1 on April 23, 2026, filed May 31, 2024, for a single-chain fragment variable (scFv) targeting KRAS G12V and a chimeric antigen receptor (CAR). The invention modifies a T cell receptor to recognize KRAS G12V mutant polypeptides presented by HLA-A*02:01, retaining the extracellular signaling domain linked to a CD3ζ-derived intracellular signaling domain for potential tumor treatment applications including CAR-T and NK cell therapy.

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GovPing monitors USPTO Patent Applications - Therapeutics (A61P) for new healthcare & life sciences regulatory changes. Every update since tracking began is archived, classified, and available as free RSS or email alerts — 219 changes logged to date.

What changed

USPTO published patent application US20260108609A1 for a single-chain fragment variable (scFv) targeting the KRAS G12V mutation and a corresponding chimeric antigen receptor (CAR). The application describes modifying a T cell receptor to specifically recognize KRAS G12V mutant polypeptides presented by HLA-A*02:01, retaining the extracellular signaling domain and linking it to a CD3ζ-derived intracellular signaling domain. The inventors, Zhenyu XU and Huimin SHAO, disclose applications in CAR-T and NK cell therapies for potential cancer treatment.

Affected parties in the biotechnology, pharmaceutical, and oncology sectors should monitor this published application to assess potential freedom-to-operate considerations and competitive landscape impacts related to KRAS G12V-targeted cell therapies.

Archived snapshot

Apr 23, 2026

GovPing captured this document from the original source. If the source has since changed or been removed, this is the text as it existed at that time.

← USPTO Patent Applications

SINGLE-CHAIN FRAGMENT VARIABLE TARGETING KRAS G12V, CHIMERIC ANTIGEN RECEPTOR, AND USE THEREOF

Application US20260108609A1 Kind: A1 Apr 23, 2026

Inventors

Zhenyu XU, Huimin SHAO

Abstract

Disclosed are a single-chain fragment variable (scFv) targeting KRAS G12V, a chimeric antigen receptor (CAR), and a use thereof. Based on the KRAS G12V target, a T cell receptor (TCR) is modified. An extracellular signaling domain of the TCR for recognizing a tumor-specific antigen (TSA) is retained and linked in series to an extracellular spacer, a transmembrane domain, and a CD3ζ-derived intracellular signaling domain in the conventional CAR structure, such that the modified CAR can specifically recognize a KRAS G12V mutant polypeptide presented by HLA-A*02:01. Moreover, based on the advantages of CAR-T cell/NK cell therapy, potential new tumor treatment options are explored to lay a foundation for clinical trials.

CPC Classifications

A61K 40/4253 A61K 40/11 A61K 40/31 A61P 35/00 C07K 14/7051 C07K 16/40 C07K 2317/569

Filing Date

2024-05-31

Application No.

19132969

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Last updated

Classification

Agency
USPTO
Instrument
Notice
Branch
Executive
Legal weight
Non-binding
Stage
Final
Change scope
Minor

Who this affects

Applies to
Pharmaceutical companies Biotechnology companies
Industry sector
3254.1 Biotechnology
Activity scope
Patent application Biotechnology research
Geographic scope
United States US

Taxonomy

Primary area
Intellectual Property
Operational domain
Legal
Topics
Healthcare Pharmaceuticals

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