Double Tagged Serratia Marcescens Nuclease Patent Application
Summary
USPTO published patent application US20260092265A1 for a double-tagged Serratia Marcescens Nuclease (SMN) engineered with a C-terminal Chitin Binding Domain (CBD-tag) and a 6-mer poly-histidine tag connected by a Gly-Ser linker. Inventors: Dapeng Sun, Aine Quimby, Zhenyu Zhu. Application No. 19324955 was filed September 10, 2025.
What changed
USPTO published a patent application describing a recombinant Serratia Marcescens Nuclease (SMN) protein engineered to include two affinity tags at its C-terminus: a Chitin Binding Domain (CBD-tag) followed by a 6-mer poly-histidine tag separated by a Gly-Ser linker. The dual-tag configuration enables the enzyme to be easily removed from reaction solutions following nucleic acid digestion or bound to solid supports for sample processing. CPC classifications include C12N 9/16, C08B 37/003, and C12Y 301/30002, indicating applications in enzyme technology and carbohydrate biochemistry.
Patent applications do not impose compliance obligations on third parties. Companies engaged in nucleic acid digestion, enzyme purification, or recombinant protein manufacturing should monitor this application's prosecution to assess potential freedom-to-operate implications for related technologies. No action is required at this stage beyond routine IP portfolio review.
Archived snapshot
Apr 2, 2026GovPing captured this document from the original source. If the source has since changed or been removed, this is the text as it existed at that time.
Double Tagged Serratia Marcescens Nuclease
Application US20260092265A1 Kind: A1 Apr 02, 2026
Inventors
Dapeng Sun, Aine Quimby, Zhenyu Zhu
Abstract
Either wild type or mutant Serratia Marcescens Nuclease (“SMN”) is engineered to display a C-terminal Chitin Binding Domain (CBD-tag), followed, at the C-terminus side of the CBD-tag, by a poly-histidine tag (His-tag), where the His-tag is preferably a 6-mer and preferably is preceded by a Gly-Ser linker, thereby generating a recombinant SMN protein that retains dual affinity tags (a CBD-tag and a His-tag) at the C-terminus, to make it easily removed from a reaction solution following digestion of nucleic acids in the reaction mixture. It can also be used for binding SMN to a solid support for use in nucleic acid digestion in a sample contacted with the solid support.
CPC Classifications
C12N 9/16 C08B 37/003 C12Y 301/30002
Filing Date
2025-09-10
Application No.
19324955
Named provisions
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