CRISPR/Cas13a Kit for Rapid ALV-J Detection
Summary
The USPTO has published a patent application for a CRISPR/Cas13a kit designed for the rapid detection of avian leukosis virus subgroup J (ALV-J). This application details a method combining CRISPR/Cas13a with recombinase aided amplification for improved sensitivity and specificity in detecting ALV-J.
What changed
This document is a patent application (US20260085369A1) filed with the USPTO for a kit and method to rapidly detect avian leukosis virus subgroup J (ALV-J) using a CRISPR/Cas13a system. The described method combines CRISPR/Cas13a with recombinase aided amplification (RAA) to enhance detection sensitivity and specificity, differentiating ALV-J from other avian viruses. The application highlights the method's efficiency, low equipment requirements, and suitability for on-site detection.
This patent application does not impose immediate regulatory obligations on entities. However, it signifies potential future developments in diagnostic tools for avian diseases. Companies involved in veterinary diagnostics, biotechnology, or animal health may find this technology relevant for research and development, potentially leading to new commercial products or diagnostic services in the future.
Archived snapshot
Mar 27, 2026GovPing captured this document from the original source. If the source has since changed or been removed, this is the text as it existed at that time.
KIT FOR RAPID DETECTION OF AVIAN LEUKOSIS VIRUS SUBGROUP J BASED ON CRISPR/Cas13a SYSTEM
Application US20260085369A1 Kind: A1 Mar 26, 2026
Inventors
Xi LAN, Qigui WANG, Haiwei WANG, Shutao CHEN, Min TAN, Zhifu CUI, Yongju ZHAO
Abstract
Provided is a kit for rapid detection of avian leukosis virus subgroup J (ALV-J) based on a CRISPR/Cas13a system. The method is based on the combination of the CRISPR/Cas13a system and recombinase aided amplification (RAA) for ALV-J detection. An oligonucleotide probe is designed as a substrate for CRISPR/Cas13a trans-cleavage and produces a detectable signal. The method can substantially improve detection sensitivity by amplifying a detection signal twice by RAA and T7 transcription. The detection method further exhibits excellent specificity, allowing for clear differentiate from other avian viruses. It does not require expensive experimental equipment and special laboratory environment, and it is rapid and efficient. The method is of great significance for biological research and on-site detection of ALV-J.
CPC Classifications
C12Q 1/702 C12N 9/22 C12N 15/11 C12N 2310/20 C12N 2320/10 C12Q 2600/158
Filing Date
2025-03-14
Application No.
19080176
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