Methods and systems for extracting nucleic acids from a biological sample

Abstract

A method for extracting nucleic acids includes mixing a biological sample with a solid-phase substrate to produce a sample fluid. The nucleic acids in the sample fluid bind to the solid-phase substrate. The method also includes flowing the sample fluid in a fluid conduit to a trapping site. The trapping site may include a chamber. The method may further include applying a magnetic field to trap the solid-phase substrate of the sample fluid flowing through the fluid conduit at the trapping site. The method further includes flowing a wash buffer through the fluid conduit to remove impurities from the solid-phase substrate. The method further includes flowing an immiscible fluid through the fluid conduit to remove residual sample fluid and/or wash buffer. The method further includes flowing an elution buffer through the fluid conduit to elute nucleic acids from the solid-phase substrate.

CPC Classifications