← USPTO Patent Grants

Protein production method and cell-free protein synthesis kit

Grant US12577601B2 Kind: B2 Mar 17, 2026

Assignee

TAIYO NIPPON SANSO CORPORATION

Inventors

Takashi Yabuki, Takanori Kigawa, Kae Higuchi

Abstract

The object of the present invention is to provide a protein production method capable of producing an active protein with high efficiency even at a low temperature, and a cell-free protein synthesis kit. A protein production method including producing a protein with a reaction solution of a cell-free protein synthesis system containing either one or both of a cold shock protein and a nucleic acid containing a coding region encoding an amino acid sequence of the cold shock protein. A cell-free protein synthesis kit including one or both of a cold shock protein and a nucleic acid containing a coding region encoding an amino acid sequence of the cold shock protein, and a reaction solution of a cell-free protein synthesis system.

CPC Classifications

C12P 21/02 C12N 1/20 C12N 15/11 C12N 2500/32 C12N 2501/998 C12N 2800/10 C12R 2001/01 C12R 2001/125 C12R 2001/19 C07K 14/195 C07K 14/245 C07K 14/32

Filing Date

2020-07-16

Application No.

17627233

Claims

9