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Orthogonal Cas9 proteins for RNA-guided gene regulation and editing

Grant US12577590B2 Kind: B2 Mar 17, 2026

Assignee

President and Fellows of Harvard College

Inventors

George M. Church, Kevin M. Esvelt, Prashant G. Mali

Abstract

Methods of modulating expression of a target nucleic acid in a cell are provided including use of multiple orthogonal Cas9 proteins to simultaneously and independently regulate corresponding genes or simultaneously and independently edit corresponding genes.

CPC Classifications

C12N 15/907 C12N 9/22 C12N 15/11 C12N 15/113 C12N 15/85 C12N 2310/3519 C12N 2320/33 C12N 2320/50 C12N 2800/107 C12N 2800/40 C12N 2800/80 C12N 2810/10 C12N 2830/00 C12N 2830/34 C12N 2999/007 C07K 2/00 C12Y 301/00

Filing Date

2023-04-06

Application No.

18296446

Claims

14