Synthetic production of single-stranded adeno associated viral DNA vectors
Grant
US12577573B2
Kind: B2
Mar 17, 2026
Assignee
Generation Bio Co.
Inventors
Ozan Alkan, Robert Michael Kotin, Douglas Anthony Kerr, Russell Monds, Carolyn Pelletier, Matthew Stanton
Abstract
The present application discloses methods for synthetic production and cell-free synthesis of single stranded adeno-associated virus (AAV) vectors, for delivery and expression of a transgene in host cells. The present invention also relates to an in vitro process for production of closed-ended DNA vectors and corresponding single stranded AAV DNA vector products synthesized from the closed-ended DNA vectors having nicks.
CPC Classifications
C12N 15/66-69
Filing Date
2020-07-17
Application No.
17617332
Claims
23