CELL ADHESION SYSTEM FOR INTERSPECIES CHIMERAS

Abstract

Disclosures herein are directed to populations of cells comprising one or more cell of a first mammalian species and one or more cell of a second mammalian species, such as stem cells and embryos, wherein the cells are engineered to express an either an antigen-binding protein or an antigen, wherein each are expressed with a cell membrane signaling peptide and/or a cell membrane anchor protein. The inclusion of the cell membrane signaling peptide or a cell membrane anchor protein enhances cell adhesion, enabling improved formation of interspecies chimeras, for example the generation of a human and non-human interspecies chimera. Also provided herein are methods of manufacturing the population of cells as described herein. Also provided are methods of organogenesis to generate an organ, organoid, or tissue mass comprising an interspecies chimeric animal for organ transplant, and methods of treatment using the same.

CPC Classifications