Cell Based Assays for Botulinum

Abstract

Serotype-specific cell based assays for the characterization of Clostridia botulinum neurotoxins and genetically modified cells utilized in such assays are described. Such assays and genetically modified cells can discriminate between different serotypes of clostridium neurotoxins having the same SNARE protein substrate specificity, despite a lack of such discrimination in the parental cell line. This discrimination is achieved without the use of serotype-specific neutralizing antibodies and without the use of competing heavy chains derived from a Clostridia botulinum neurotoxin.

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