DESIGN OF SELECTIVELY TRANSLATED mRNAS

Abstract

Methods of designing a synthetic RNA molecule comprising obtaining a coding sequence encoding a protein of interest; obtaining a sequence of an RNA expressed in a first tissue or cell type and not expressed or lowly expressed in a second tissue or cell type; selecting a region within the sequence of an RNA; and producing a sequence of a synthetic RNA molecule comprising from 5′ to 3′: a reverse complement to the selected region comprising a 5′ unhybridized region and a 3′ hybridized region, a loop region that does not hybridize; a region reverse complementary to 3′ hybridized region and the obtained coding sequence encoding a protein of interest are provided. Synthetic RNA molecules produced by a method of the invention and computer program products for perform a method of the invention are also provided.

CPC Classifications