METHOD OF DETECTING A POLYNUCLEOTIDE ANALYTE
Application
US20260085344A1
Kind: A1
Mar 26, 2026
Inventors
Matthew Wook Chang, Nikhil Aggarwal, In Young Hwang, Hua Ling, Jee Loon Foo
Abstract
The invention relates generally to the field of nucleic acid detection. In particular, the specification teaches a method of detecting a polynucleotide analyte in a sample. In one aspect, the method comprises the use of a cleaving agent with flap endonuclease activity and a type V CRISPR/Cas effector protein. In another aspect, the type V CRISPR/Cas effector protein is a Cast 2 protein.
CPC Classifications
C12Q 1/6823
C12N 9/226
C12N 15/113
C12Q 1/25
C12Q 1/34
C12Q 1/48
C12Q 1/6844
C12Q 1/6876
C12Y 207/07007
C12Y 600/00
C12N 2310/20
C12Q 2600/156
Filing Date
2023-04-14
Application No.
18856243