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Compositions for cDNA Synthesis and Transcriptome Profiling and Methods of Use Thereof

Application US20260085309A1 Kind: A1 Mar 26, 2026

Inventors

Anna Marie Pyle, Li-Tao Guo

Abstract

The present invention provides optimized template switching oligonucleotides, methods, and kits for performing reverse transcription. The optimized template switching oligonucleotides include modifications of 5′ and 3′ ends to prevent the formation of concatemers and to enhance the specificity of reverse transcription.

CPC Classifications

C12N 15/1096 C12N 9/1276 C12P 19/34 C12Q 1/6848 C12Y 207/07049

Filing Date

2023-09-14

Application No.

19111674