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COMPOSITIONS AND METHODS FOR MODIFYING GENOMES

Application US20260085301A1 Kind: A1 Mar 26, 2026

Assignee

Confluence Genetics, LLC

Inventors

Matthew Brett Begemann, Emma Elizabeth January, Gina C. Neumann, Allison Jane Newton Antonakos, Anna Singer, Erin Zess

Abstract

Compositions and methods for targeting pre-determined DNA sequences in cells of interest are provided. The methods result in the targeted elimination of cells that comprise the pre-determined DNA sequence(s). Compositions comprise DNA constructs comprising nucleotide sequences that encode a Cas12a2 protein operably linked to a promoter that is operable in the cells of interest. Methods to use these DNA constructs to selectively target and eliminate cells that harbor the targeted DNA sequence(s) are described herein.

CPC Classifications

C12N 9/226 C12N 15/11 C12N 15/8213 C12N 15/86 C12N 15/902 C12N 15/907 C12N 2310/20 C12N 2750/14143 C12N 2800/80

Filing Date

2025-09-19

Application No.

19334505