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CELL CULTURE METHOD AND PRODUCTION METHOD FOR PRODUCT

Application US20260085282A1 Kind: A1 Mar 26, 2026

Assignee

FUJIFILM Corporation

Inventors

Atsushi INADA, Shinichi NAKAI, Katsuhiro SHIMONO

Abstract

An object of the present invention is to provide a cell culture method and a production method for a product, in which an occurrence of aggregates in foam is suppressed. According to the present invention, there is provided a cell culture method of culturing cells at a cell density of 30×106 cells/mL or more and 400×106 cells/mL or less in a culture solution, the method including: step A of adjusting a poloxamer concentration in a foam liquid constituting foam to be 6 times or less a poloxamer concentration in the culture solution; or step B of adjusting a viscosity of the foam liquid constituting the foam to be 1.5 mPa·s or less under conditions of 35° C. or higher and 38° C. or lower.

CPC Classifications

C12N 5/0018 C07K 16/00 C12N 2500/50

Filing Date

2025-11-28

Application No.

19403607