CONTINUOUS GRADIENT ELUTION CHROMATOGRAPHIC FRACTIONATION

Abstract

The present invention relates to a method for separating a product of interest from impurities and an apparatus for performing the method. The method comprises the steps in the indicated order: (1) loading on a chromatography matrix a first volume of a feed solution comprising the product of interest and impurities; (2) contacting the chromatography matrix with an elution solution; (3) collecting a) optionally an elution fraction 1 (EF1) in a side fraction container (SFC), b) an elution fraction 2 (EF2) in a product container, and c) optionally an elution fraction 3 (EF3) in a SFC, wherein at least one of EF1 and EF3 is collected; (4) loading on a chromatography matrix EF1 and/or EF3 and a second volume of the feed solution simultaneously or subsequently. Steps (2) to (4) are repeated at least once, and the chromatography matrices of step (1) and (4) are the same or different.

CPC Classifications