RECOMBINANT HUMAN TYPE III COLLAGEN FOR PROMOTING ANGIOGENESIS, AND PREPARATION METHOD AND APPLICATION METHOD THEREOF

Abstract

Recombinant human type III collagen for promoting angiogenesis, and a preparation method and an application method thereof are provided, which relate to the field of genetic engineering technologies. The amino acid of the recombinant human type III collagen is as shown in SEQ ID NO: 1. The method replaces nine different sites of a full-length natural type III collagen with a GFOGER sequence from type I collagen specifically binding to integrin sites, thereby enhancing promote angiogenic function without changing a total length of natural full-length amino acids. Meanwhile, the method replaces four natural integrin sites with lower adhesion rates, thus the recombinant type III collagen have higher adhesion, to further promote angiogenesis. Through this modification method, the angiogenesis rate will be increased by more than 37%, and the molecular weight of protein translated by the recombinant human type III collagen is moderate, making it easy to prepare.

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