METHOD FOR PREPARING OLIGONUCLEOTIDE BY USING RNA LIGASE
Application
US20260078424A1
Kind: A1
Mar 19, 2026
Inventors
Hao Hong, Gage James, Na Zhang, Xuecheng Jiao, Zhaoshuai Wang, Fang Liu, Cuiping Ma, Yuhan Geng, Yiming Yang, Juan Li, Lixin Cui, Wenxuan Zhu, Xu Jia
Abstract
Provided is a method for preparing an oligonucleotide with a ribonucleic acid (RNA) ligase. The RNA ligase includes any one or more enzymes of RNA ligase families Rnll, Rn12, Rn13, and Rn15, and the oligonucleotide includes natural RNA or non-natural RNA. The method may solve the problem that in the prior art, it is difficult to efficiently synthesize a non-natural RNA strand, and the method is suitable for the field of RNA synthesis.
CPC Classifications
C12P 19/34
C12N 9/16
C12N 9/93
C12Y 301/21001
C12Y 605/01003
Filing Date
2023-03-17
Application No.
19109742