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ORTHOGONAL METABOLIC FRAMEWORK FOR ONE-CARBON UTILIZATION

Application US20260078416A1 Kind: A1 Mar 19, 2026

Inventors

Ramon Gonzalez, Alexander Chou, James MacAllister Clomburg, Fayin Zhu, Seung Hwan Lee, Mohammadreza Nezamirad

Abstract

Provided are systems and methods for converting C1 substrates to products contain more than one carbon, without producing central metabolic building blocks as intermediate products. In an embodiment, system/method can include a biochemical pathway enabling an orthogonal platform for C1 utilization based on formyl-CoA elongation (FORCE) reactions. In an embodiment, the system/method can include acyloin condensations between formyl-CoA and carbonyl-containing molecules. In an embodiment, the system/method can include a reactions catalyzed by the enzyme 2-hydroxyacyl-CoA lyase (HACL).

CPC Classifications

C12P 7/42 C12N 9/1025 C12N 9/1029 C12N 9/88 C12N 9/93 C12N 15/70 C12P 7/56 C12Y 203/01023 C12Y 401/01008 C12Y 602/01003 C12N 2800/101

Filing Date

2022-08-16

Application No.

18442550