SCARLESS GENOME EDITING THROUGH TWO-STEP HOMOLOGY DIRECTED REPAIR
Application
US20260078401A1
Kind: A1
Mar 19, 2026
Assignee
The Board of Trustees of the Leland Stanford Junior University
Inventors
Kazuya Ikeda, Matthew H. Porteus
Abstract
A method for scarless genome editing is disclosed. In particular, the method provides scarless genome modification by using homology directed repair (HDR) steps to genetically modify cells and remove unwanted sequences. This method can be used for genome editing, including introducing mutations, deletions, or insertions at any position in the genome without leaving silent mutations, selection marker sequences, or other additional undesired sequences in the genome.
CPC Classifications
C12N 15/85
C12N 9/22
C12N 15/902
C12N 15/907
G01N 21/6486
A61K 48/00
C12N 5/0606
C12N 5/0607
C12N 2310/122
C12N 2310/531
C12N 2510/04
Filing Date
2025-09-22
Application No.
19336224