METHOD FOR EFFICIENT GENETIC TRANSFORMATION AND GENE EDITING OF BRASSICA CROP MEDIATED BY AGROBACTERIUM RHIZOGENES

Abstract

A method for efficient genetic transformation and gene editing of a Brassica crop mediated by Agrobacterium rhizogenes is provided. Genetic transformation of the Brassica crop and establishment of a gene editing system are achieved by Agrobacterium rhizogenes-mediated delivery of three developmental regulators (DRs), ZmWUS2, AtIPT, and AtPLT5. The Agrobacterium rhizogenes-mediated delivery of three DRs, ZmWUS2, AtIPT, and AtPLT5, may induce explants to form callus and then directly form buds. The method solves the problem of difficult root regeneration for the Brassica crop, and enables species with a poor root regeneration ability to achieve efficient transformation.

CPC Classifications