SYSTEMS, METHODS, AND COMPOSITIONS FOR AN INDUCIBLE PROMOTER SYSTEM

Abstract

In variants, an inducible promoter system, can include: a gene of interest; an operator site; a first nucleotide sequence encoding a reverse cumate transcriptional activator; and a second nucleotide sequence encoding a cumate repressor. In a specific example, the cumate repressor can bind to the operator site to repress transcription of the gene of interest and the reverse transcriptional activator can bind to the operator site in the presence of an activator to activate transcription of the gene of interest.

CPC Classifications