RECOMBINANT CARBONIC ANHYDRASE POLYPEPTIDES AND METHODS OF USE THEREOF

Abstract

Disclosed herein are recombinant carbonic anhydrase polypeptide comprising an amino acid sequence having one or more amino acid modification as compared to SEQ ID NO: 13, wherein amino acid residues W32, Y34, G36, E37, G39, P40, W43, L46, E49, C53, K56, N57, Q58, P60, V61, A71, L73, L76, N79, Y80, 188, N90, N91, G92, H93, T94, V97, G109, L114, K115, Q116, F117, H118, F119, H120, A121, P122, S123, E124, G129, Y132, P133, E135, H137, V139, H140, D142, K143, D144, G145, N146, A148, V149, V152, F154, K155, E156, G157, N160, G175, N184, P190, Y195, Y196, S199, G200, D201, L202, T203, T24, P205, P206, C207, E209, G210, V211, W213, I214, V215, K217, S223, K224, Q226, I227, F230, M234, N239, R240, P241, Q243, P244, N246, R248, and/or I250, are unmodified, and variants thereof. Methods for use and kits are also disclosed herein.

CPC Classifications