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DNA POLYMERASE MUTANT FOR SEQUENCING

Application US20260078358A1 Kind: A1 Mar 19, 2026

Assignee

WUHAN MGI TECH CO., LTD.

Inventors

Fen LIU, Siqian HUANG, Liyuan YANG, Jing GAO, Jiakun BAI, Yang XIAO, Juan YANG, Xiang SU, Yuepeng LIU, Chengwei SONG

Abstract

Provided is a DNA polymerase mutant for sequencing. The DNA polymerase mutant includes: compared with a Pyrococcus abyssi DNA polymerase exo-mutant, at least three amino acid mutations in the following four sites or functionally equivalent sites: position 409, position 410, position 411, and position 486. The Pyrococcus abyssi DNA polymerase exo-mutant has an amino acid sequence as set forth in SEQ ID NO: 1.

CPC Classifications

C12N 9/1252 C12N 1/20 C12N 15/70 C12P 19/34 C12Q 1/6869 C12R 2001/19 C12Y 207/07007

Filing Date

2025-11-26

Application No.

19401896