KETOREDUCTASE ENZYMES FOR THE SYNTHESIS OF 1,3-DIOL SUBSTITUTED INDANES
Application
US20260078354A1
Kind: A1
Mar 19, 2026
Assignee
Merck Sharp & Dohme LLC
Inventors
Jackson Kenai Blender Cahn, Wai Ling Cheung-Lee, Stephanie W. Chun, Kaori Hiraga, Birgit Kosjek, John Knight, Amanda M. Makarewicz, Scott McCann, Jeffrey C. Moore, Deeptak Verma
Abstract
The present disclosure provides ketoreductase enzymes having improved enzymatic properties including the capability of reducing hydroxy indanones to provide diastereomerically pure 1,3-indane diols useful for the synthesis of belzutifan. Also provided are polynucleotides encoding the ketoreductase enzymes, and host cells capable of expressing the ketoreductase enzymes. A purification procedure for isolating the ketoreductase enzymes is also provided.
CPC Classifications
C12N 9/0006
C12N 15/70
C12P 11/00
C12R 2001/19
C12Y 101/01
Filing Date
2023-07-05
Application No.
18880970