ATP-INDEPENDENT BIOLUMINESCENT REPORTER VARIANTS TO IMPROVE IN VIVO IMAGING

Abstract

Disclosed are ATP-independent bioluminescent reporter systems for enhanced in vivo imaging. Also disclosed is a modified luciferin, potassiorin, comprising a potassium-binding moiety. Also disclosed is an engineered luciferase, BRIPO, optimized for synergistic interaction with potassiorin. A further version of the disclosed system produces bioluminescence signals responsive to physiological potassium concentrations. In further instances, the system enables real-time monitoring of K+ dynamics in live cells, tissues, and animals. A further embodiment includes applications in imaging neuronal activity, studying ion flux, and developing bioluminescent indicators for diverse analytes. The disclosed system addresses limitations of traditional imaging methods, offering improved sensitivity and biocompatibility.

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