METHOD FOR PURIFYING ANTIBODY HAVING LOW ISOELECTRIC POINT
Applicants
CHUGAI SEIYAKU KABUSHIKI KAISHA
Inventors
UEDA, Yasufumi, KOBAYASHI, Shohei, YANAGITA, Satoko, KAWASE, Takuo, FUKUNAGA, Masahiro
Abstract
The present inventors discovered that additional aggregation of low-pI antibody can be suppressed by removing formed antibody aggregates after a certain period of time following Protein A column purification, acidic treatment, and neutralization. Furthermore, the present inventors found that efficient impurities removal for a low-pI antibody can be accomplished by using an anion exchange resin in the Bind/Elute mode and then a hydrophobic interaction chromatography or multimodal chromatography resin, compared with conventional methods.
IPC Classifications
Designated States
AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LI, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR