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METHOD FOR INDUCING DIFFERENTIATION INTO PLURIPOTENT STEM CELLS- DERIVED CONJUNCTIVAL CELLS

Publication EP3783097A1 Kind: A1 Mar 18, 2026

Applicants

Osaka University

Inventors

NISHIDA, Kohji, HAYASHI, Ryuhei, NOMI, Kimihito

Abstract

Provided is a method for inducing differentiation into conjunctival epithelial cells, conjunctival goblet cells, and conjunctival epithelial stem and progenitor cells, the method comprising culturing colonies of pluripotent stem cells in a medium containing an epidermal growth factor (EGF) signaling activator to induce differentiation of the pluripotent stem cells. The present invention enables differentiation of pluripotent stem cells including iPS cells into conjunctival epithelial cells, conjunctival goblet cells, and conjunctival epithelial stem and progenitor cells and therefore is very useful for, for example, basic research on conjunctival epithelial cells and conjunctival goblet cells, regenerative therapy for intractable ocular surface diseases, and research related to the diseases.

IPC Classifications

C12N 5/079 20100101AFI20251128BHEP G01N 33/50 20060101ALI20251128BHEP

Designated States

AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LI, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR