METHOD FOR PRODUCING CELL MASS INCLUDING PITUITARY TISSUE, AND CELL MASS THEREOF

Publication EP3715450A1 Kind: A1 Mar 18, 2026

Applicants

Sumitomo Chemical Company, Limited

Inventors

NAKANO, Tokushige

Abstract

The present invention aims to provide a method for efficiently producing a cell mass containing pituitary tissue from pluripotent stem cells. A method for producing a cell mass containing pituitary tissue, including the following steps (1) and (2):(1) a first step of suspension-culturing pluripotent stem cells to form a cell aggregate in the presence of a Wnt signal transduction pathway inhibiting substance,(2) a second step of suspension-culturing the aggregate obtained in the first step in the presence of a BMP signal transduction pathway activating substance and a Sonic hedgehog signal transduction pathway activating substance, thereby obtaining a cell mass comprising pituitary tissue.

IPC Classifications

C12N 5/0735 20100101AFI20210721BHEP A61K 35/30 20150101ALI20210721BHEP A61P 5/06 20060101ALI20210721BHEP A61P 5/10 20060101ALI20210721BHEP A61P 43/00 20060101ALI20210721BHEP C12N 5/079 20100101ALI20210721BHEP C12N 5/10 20060101ALI20210721BHEP C12N 5/071 20100101ALI20210721BHEP C12N 5/077 20100101ALI20210721BHEP

Designated States

AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LI, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR

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