METHOD FOR INCREASING SECRETION OF RECOMBINANT PROTEIN
Publication
EP3680343A2
Kind: A2
Mar 18, 2026
Applicants
Korea Advanced Institute of Science and Technology
Inventors
AHN, Jung Hoon, BYUN, Hyunjong, PARK, Jiyeon
Abstract
The present invention provides a method for effective extracellular secretion of a target protein, by preparing a fusion protein connecting LARD3 to the target protein and having pI lowered by adjusting the overall charge of target protein, and by using ABC transporter of a bacterial type 1 secretion system (T1SS). The method can allows a protein be produced at a large amount simply and effectively without a separate purification process.
IPC Classifications
C12P 21/00 20060101AFI20201007BHEP
C12N 9/24 20060101ALI20201007BHEP
C07K 14/415 20060101ALI20201007BHEP
Designated States
AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LI, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR