PRODUCTION OF RECOMBINANT VACCINE AGAINST HELMINTHS IN PICHIA PASTORIS
Applicants
Tendler, Miriam
Inventors
SIMPSON, Andrew J G, RAMOS, Celso Raul Romero Ramos, TENDLER, Miriam
Abstract
The present invention is related to the recombinant production of proteins by using a synthetic gene for high protein expression inPichia pastoris.More specifically, the invention describes the production of Sm14Schistosoma mansonirecombinant protein, where a synthetic gene was created to promote high expression of such protein, a gene which was cloned under control of two types ofPichia pastorispromoters: methanol-inducible promoter (AOXI) and constituent promoter (GAP). With these constructions,Pichia pastorisstrains were genetically manipulated to efficiently produce vaccine antigen Sm14. The processes to produce and purify this protein fromP. pastoriscells, which can be escalated for their industrial production, were also improved.
IPC Classifications
Designated States
AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LI, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR